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| model features |
cancer pathogenesis |
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| | > | | | Cell-cell adhesion is defined by the qualitative analysis of the phenotype of aggregated cells plated on a substrate that only allows cell-cell adhesion. |
| | > | | | Cell-matrix adhesion is defined by the quantification of adherent cells on a chosen matrix protein. |
| | > | | | Cell migration is measured by the quantification of migrating cells using 3 different methods: wound-healing, Boyden chamber, and/or videomicroscopy assays. |
| | (1) | The wound-healing assay allows directional migration (mainly involving cell-cell adhesion proteins) to be followed. |
| | (2) | The Boyden chamber assay allows directional migration to be followed (haptotaxy or chemotaxy) through a porous filter in which cell-matrix adhesion proteins are mainly involved. |
| | (3) | The videomicroscopy assay allows measurement of distance and speed of directional and random migration. |
| | > | | | Cell invasion is defined by the quantification of invasive cells in 3D matrix gel. This assay includes 3 processes: adhesion, enzymatic degradation of extracellular matrix and cell motility. |
| | > | | | These systems also allow identification of the molecules involved. |
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