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| schema of model features |
internalisation of receptors |
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culture cell model |
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| in vitro experiments |
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| molecular analysis |
cellular analysis |
IHC analysis |
mRNA
DNA synthesis
cytotoxicity
FACS
ELISA tests |
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immortalised cell lines
primary cell cultures
cell function assays |
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| confocal microscopy, cellular localisation (target cell) and subcellular localisation (nucleus, endosomes…) of biomarkers, colocalisation, quantitative analysis of biomarkers, GPCR internalisation, cellular trafficking... |
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