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biomarker colocalisation wound-healing assay



A  
B  
C  
 
Illustration of colocalisation of alanyl aminopeptidase (CD13) in the apical region of differentiated epithelial cells.

A: phalloidine-TRITC labelling of the cell membrane (in red)


B: immunolabelling of CD13 (in blue)


C: colocalisation of phalloidine and CD13 (in white)

Note: the presence of CD13 on the cell membrane demonstrates the differentiation of cells in polarised functional intestinal epithelium.