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| model features |
wound-healing assay |
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| | > | | | Our model is composed of a homogeneous monolayer of human (as well as animal) epithelial cells. They are selected for their intrinsic properties in accordance with the type of physiological process under investigation. |
| | > | | | The cells are spread out on culture plates until they form a monolayer of differentiated cells. The epithelium is manually wounded in a given zone and specific analyses of the wound are carried out in a pre-determined time period to define the characteristics of the restoration processes: |
| | > | | | cellular migration is defined by the quantification of migrating cells and measurement of the area colonised |
| | > | | | cellular proliferation is determined by the quantification of cells incorporating cell markers of proliferation (BrdU…) |
| | > | | | cellular differentiation is defined by the distribution and quantification of the expression of apical and baso-lateral polarisation markers (isomaltase sucrase, dipeptidylpeptidase IV, GLUT5, alkaline phosphatase…) as well as transepithelial resistance measurement |
| | > | | | Investigations are carried out in vitro on differentiated epithelium. Candidates are added to the culture cells which are then treated and analysed according to the specific process chosen: immunofluorescence, biochemistry, molecular biology. |
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